col3  (Proteintech)

Journal: Acta Cirúrgica Brasileira

Article Title: Type I and type III collagen immunoexpression in rabbit skin biopsy samples treated with rosuvastatin gel and autologous platelet-rich plasma

doi: 10.1590/acb402725

Figure Lengend Snippet: Immunohistochemical analysis of the lesions. (a) Immunohistochemical staining for type I collagen on day 17 in the NC and the different treatments. Magnification: 400x. Scale bar: 50 μm. (b) Immunohistochemical staining for type III collagen on day 17 in the NC and the different treatments.

Article Snippet: Antibodies for evaluating collagen immunoexpression in rabbit wounds treated with aPRP, RSV, and aPRP + RSV included: anti-type I collagen primary antibody (1:50, mouse monoclonal, GeneTex, GTX26308), anti-type III collagen primary antibody (1:100, mouse monoclonal, Novus Biologicals, NBP1-05119), and goat anti-mouse IgG H&L (HRP) antibody (1:200, goat, Abcam, ab6789).

Techniques: Immunohistochemical staining, Staining

Journal: Acta Cirúrgica Brasileira

Article Title: Type I and type III collagen immunoexpression in rabbit skin biopsy samples treated with rosuvastatin gel and autologous platelet-rich plasma

doi: 10.1590/acb402725

Figure Lengend Snippet: Plots representing the average percentage of immunostaining for types I and III collagen under different treatments on day 17.

Article Snippet: Antibodies for evaluating collagen immunoexpression in rabbit wounds treated with aPRP, RSV, and aPRP + RSV included: anti-type I collagen primary antibody (1:50, mouse monoclonal, GeneTex, GTX26308), anti-type III collagen primary antibody (1:100, mouse monoclonal, Novus Biologicals, NBP1-05119), and goat anti-mouse IgG H&L (HRP) antibody (1:200, goat, Abcam, ab6789).

Techniques: Immunostaining

Journal: Scientific reports

Article Title: CDX1 improves nicotine induced cardiac fibroblasts activation and cardiomyocyte hypertrophy by alleviating autophagic flux impairment through modulation of LAPTM4B.

doi: 10.1038/s41598-025-94160-5

Figure Lengend Snippet: Fig. 1. Nicotine causes activation of CFs and hypertrophy of NRVMs by inducing autophagic flux blockade. The mRNA expression of myocardial fibrosis markers Col1, Col3, α-SMA, FN (A) and myocardial hypertrophy markers ANP, BNP, β-MHC (C) were detected by qPCR. The protein expression of myocardial fibrosis markers was detected by Western blot (WB) (B). Expression of autophagy marker LC3II and its specific substrate p62 in nicotine-stimulated CFs was detected by WB (D). The mRNA level of CDX1 was detected by qPCR in CFs stimulated with different concentrations of nicotine (E), and the protein expression level of CDX1 was detected by WB (F). Results are given as mean ± SD, P value was determined by one-way AVONA with Bonferroni test, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05, n = 3.

Article Snippet: NRVMs and CFs were transfected with recombinant adenovirus encoding mCherry- Antibodies Target antigen Vendor or source Catalog # Working concentration phosphor-4EBP1 Cell signaling technology 2855s 1:1000 WB phosphor-p70s6k Cell signaling technology 9204s 1:1000 WB p70s6k Cell signaling technology 2708s 1:1000 WB phosphor-mTOR Cell signaling technology 5536s 1:1000 WB α-SMA ABclonal A1011 1:1000 WB LAPTM4B abcam ab25631 1:1000 WB 4EBP1 Proteintech 60246-1-Ig 1:1000 WB mTOR Proteintech 66888-1-Ig 1:1000 WB p62 Proteintech 14600-1-AP 1:1000 WB ,LC3II Proteintech 66184-1-Ig 1:1000 WB β-actin Proteintech 66009-1-Ig 1:2000 WB Col1 Proteintech 66761-1-Ig 1:1000 WB Col3 Proteintech 22734-1-AP 1:1000 WB GAPDH Proteintech 60004-1-Ig 1:2000 WB CDX1 Sigma-Aldrich PM045 1:100 WB Table 2.

Techniques: Activation Assay, Expressing, Western Blot, Marker

Journal: Scientific reports

Article Title: CDX1 improves nicotine induced cardiac fibroblasts activation and cardiomyocyte hypertrophy by alleviating autophagic flux impairment through modulation of LAPTM4B.

doi: 10.1038/s41598-025-94160-5

Figure Lengend Snippet: Fig. 2. CDX1 is upregulated in response to nicotine, and downregulation of CDX1 exacerbates nicotine- induced activation of CFs and cardiomyocyte hypertrophy. The mRNA expression of cardiac fibrosis markers Col1, Col3, α-SMA, FN in CFs with CDX1 knockdown (siCDX1) (A) and cardiac hypertrophy markers ANP, BNP, β-MHC in NRVMs (C) were detected by applying qPCR. The protein expression of Col1, Col3, α-SMA, FN upon downregulation of CDX1 was detected by WB (B). The mRNA expression of Col1, Col3, α-SMA, FN in CFs during CDX1 overexpression (AdCDX1) (D) and the mRNA expression of ANP, BNP, β-MHC in NRVMs were detected by qPCR (F). WB was used to detect the protein expression of Col1, Col3, α-SMA, FN in AdCDX1 (E). Results are given as mean ± SD, P value was determined by two-way AVONA with Bonferroni test, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05, n = 3.

Article Snippet: NRVMs and CFs were transfected with recombinant adenovirus encoding mCherry- Antibodies Target antigen Vendor or source Catalog # Working concentration phosphor-4EBP1 Cell signaling technology 2855s 1:1000 WB phosphor-p70s6k Cell signaling technology 9204s 1:1000 WB p70s6k Cell signaling technology 2708s 1:1000 WB phosphor-mTOR Cell signaling technology 5536s 1:1000 WB α-SMA ABclonal A1011 1:1000 WB LAPTM4B abcam ab25631 1:1000 WB 4EBP1 Proteintech 60246-1-Ig 1:1000 WB mTOR Proteintech 66888-1-Ig 1:1000 WB p62 Proteintech 14600-1-AP 1:1000 WB ,LC3II Proteintech 66184-1-Ig 1:1000 WB β-actin Proteintech 66009-1-Ig 1:2000 WB Col1 Proteintech 66761-1-Ig 1:1000 WB Col3 Proteintech 22734-1-AP 1:1000 WB GAPDH Proteintech 60004-1-Ig 1:2000 WB CDX1 Sigma-Aldrich PM045 1:100 WB Table 2.

Techniques: Activation Assay, Expressing, Knockdown, Over Expression

Journal: Scientific reports

Article Title: CDX1 improves nicotine induced cardiac fibroblasts activation and cardiomyocyte hypertrophy by alleviating autophagic flux impairment through modulation of LAPTM4B.

doi: 10.1038/s41598-025-94160-5

Figure Lengend Snippet: Fig. 4. Up-regulation of CDX1 alleviates autophagic flux blockade and down-regulation of CDX1 exacerbates autophagic flux blockade. WB detection of LC3II and p62 expression in CFs after AdCDX1 stimulation by Baf A1 and nicotine alone or in combination (A). WB detection of LC3II and p62 expression in CFs after siCDX1 stimulation by Rapa and nicotine alone or in combination (B). Results are given as mean ± SD, P value was determined by two-way AVONA with Bonferroni test, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05, n = 3. Under nicotine and Baf A1 (100 nM) stimulation alone or in combination, qPCR was performed to detect mRNA expression of Col1, Col3, α-SMA and FN in CFs after CDX1 overexpression (C) and mRNA expression of ANP, BNP, β-MHC in NRVMs (D). Results are given as mean ± SD, P value was determined by one-way AVONA with Bonferroni test, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05, n = 3.

Article Snippet: NRVMs and CFs were transfected with recombinant adenovirus encoding mCherry- Antibodies Target antigen Vendor or source Catalog # Working concentration phosphor-4EBP1 Cell signaling technology 2855s 1:1000 WB phosphor-p70s6k Cell signaling technology 9204s 1:1000 WB p70s6k Cell signaling technology 2708s 1:1000 WB phosphor-mTOR Cell signaling technology 5536s 1:1000 WB α-SMA ABclonal A1011 1:1000 WB LAPTM4B abcam ab25631 1:1000 WB 4EBP1 Proteintech 60246-1-Ig 1:1000 WB mTOR Proteintech 66888-1-Ig 1:1000 WB p62 Proteintech 14600-1-AP 1:1000 WB ,LC3II Proteintech 66184-1-Ig 1:1000 WB β-actin Proteintech 66009-1-Ig 1:2000 WB Col1 Proteintech 66761-1-Ig 1:1000 WB Col3 Proteintech 22734-1-AP 1:1000 WB GAPDH Proteintech 60004-1-Ig 1:2000 WB CDX1 Sigma-Aldrich PM045 1:100 WB Table 2.

Techniques: Expressing, Over Expression

Journal: Scientific reports

Article Title: CDX1 improves nicotine induced cardiac fibroblasts activation and cardiomyocyte hypertrophy by alleviating autophagic flux impairment through modulation of LAPTM4B.

doi: 10.1038/s41598-025-94160-5

Figure Lengend Snippet: Fig. 6. Down-regulation of LAPTM4B promoted nicotine-induced activation of CFs and cardiomyocyte hypertrophy, whereas up-regulation of LAPTM4B had the opposite effect. qPCR was used to detect the mRNA expression of myocardial fibrosis markers Col1, Col3, α-SMA, FN in CFs when LAPTM4B was downregulated (A) and the mRNA expression of cardiac hypertrophy markers ANP, BNP and β-MHC in NRVMs (C). WB detected the expression of Col1, Col3, α-SMA after siLAPTM4B (B). Application of qPCR to detect the expression of Col1, Col3, α-SMA, FN in CFs (D) and the expression of ANP, BNP and β-MHC in NRVMs when LAPTM4B was overexpressed (F). WB was used to assess the protein expression levels of Col1, Col3, andα-SMA after AdLAPTM4B (E). Results are given as mean ± SD, P value was determined by two-way AVONA with Bonferroni test, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05, n = 3.

Article Snippet: NRVMs and CFs were transfected with recombinant adenovirus encoding mCherry- Antibodies Target antigen Vendor or source Catalog # Working concentration phosphor-4EBP1 Cell signaling technology 2855s 1:1000 WB phosphor-p70s6k Cell signaling technology 9204s 1:1000 WB p70s6k Cell signaling technology 2708s 1:1000 WB phosphor-mTOR Cell signaling technology 5536s 1:1000 WB α-SMA ABclonal A1011 1:1000 WB LAPTM4B abcam ab25631 1:1000 WB 4EBP1 Proteintech 60246-1-Ig 1:1000 WB mTOR Proteintech 66888-1-Ig 1:1000 WB p62 Proteintech 14600-1-AP 1:1000 WB ,LC3II Proteintech 66184-1-Ig 1:1000 WB β-actin Proteintech 66009-1-Ig 1:2000 WB Col1 Proteintech 66761-1-Ig 1:1000 WB Col3 Proteintech 22734-1-AP 1:1000 WB GAPDH Proteintech 60004-1-Ig 1:2000 WB CDX1 Sigma-Aldrich PM045 1:100 WB Table 2.

Techniques: Activation Assay, Expressing

Journal: Scientific reports

Article Title: CDX1 improves nicotine induced cardiac fibroblasts activation and cardiomyocyte hypertrophy by alleviating autophagic flux impairment through modulation of LAPTM4B.

doi: 10.1038/s41598-025-94160-5

Figure Lengend Snippet: Fig. 7. CDX1 blocks autophagic flux and induces CFs activation and cardiomyocyte hypertrophy by regulating LAPTM4B downregulation. WB detection of LC3II and p62 expression levels in LAPTM4B knockdown (A) or overexpression (B) in CFs. Results are given as mean ± SD, P value was determined by two-way AVONA with Bonferroni test, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05, n = 3. After co-transfection of CFs and NRVMs by application of AdCDX1 and siLAPTM4B, qPCR was applied to detect the expression of Col1, Col3, α-SMA, FN (C) and ANP, BNP, β-MHC (D). WB was used to detect the expression of LC3II and p62 in CFs co-transfected with AdCDX1 and siLAPTM4B (E). qPCR was used to detect the mRNA expression of LAPTM4B in CFs (G) and NRVMs (F) co-transfected with AdCDX1 and siLAPTM4B. Results are given as mean ± SD, P value was determined by one-way AVONA with Bonferroni test, **** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05, n = 3.

Article Snippet: NRVMs and CFs were transfected with recombinant adenovirus encoding mCherry- Antibodies Target antigen Vendor or source Catalog # Working concentration phosphor-4EBP1 Cell signaling technology 2855s 1:1000 WB phosphor-p70s6k Cell signaling technology 9204s 1:1000 WB p70s6k Cell signaling technology 2708s 1:1000 WB phosphor-mTOR Cell signaling technology 5536s 1:1000 WB α-SMA ABclonal A1011 1:1000 WB LAPTM4B abcam ab25631 1:1000 WB 4EBP1 Proteintech 60246-1-Ig 1:1000 WB mTOR Proteintech 66888-1-Ig 1:1000 WB p62 Proteintech 14600-1-AP 1:1000 WB ,LC3II Proteintech 66184-1-Ig 1:1000 WB β-actin Proteintech 66009-1-Ig 1:2000 WB Col1 Proteintech 66761-1-Ig 1:1000 WB Col3 Proteintech 22734-1-AP 1:1000 WB GAPDH Proteintech 60004-1-Ig 1:2000 WB CDX1 Sigma-Aldrich PM045 1:100 WB Table 2.

Techniques: Activation Assay, Expressing, Knockdown, Over Expression, Cotransfection, Transfection